Streptococcus pneumoniae serotype 33H: a novel serotype with frameshift mutations in the acetyltransferase gene wciG

Table 3 Frameshift mutations in wciG of 33G-like Pneumococci relative to wciG in 33G

Isolate	Gene size (bp)^a	Mutation	Nucleotide position^b	Amino acid change	Amino acid length^c	Predicted consequence
PMP1615	998	Single nucleotide deletion (A)	313	Amino acid 105 change from Ile to Leu	105	Frameshift generating premature stop codon at codon 106
PMP1623	989	Single nucleotide insertion (T)	163	Amino acid 55 change from Met to Tyr	95	Frameshift generating premature stop codon at codon 96
PMP1623	989	Deletion of 11 bp (ATTTTCTCACT)^d	766^d	Amino acid 55 change from Met to Tyr	95	Frameshift generating premature stop codon at codon 96

^a Size of in frame gene in 33G = 999 bp. ^b Nucleotide positions are inferred from the snippy 4.6.0 tool (https://github.com/tseemann/snippy) and are approximate because all mutations are located in homopolymeric/ambiguous regions and therefore the exact position cannot be ascertained. This also applies to all DNA alignments in supplementary data. ^c full length of WciG in 33G = 332 amino acids. ^d As the 11 bp deletion occurs within a 14 bp region flanked by ‘ACT’ on either end, it is plausible the deletion is ACTATTTTCTC starting at position 763

ISSN: 2200-6133