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Table 2 Detection of Spn in nasopharyngeal specimens from children with Spn-FLUO

From: Fluorescent antibody-based detection and ultrastructural analysis of Streptococcus pneumoniae in human sputum

Nasopharyngeal specimen

qPCR (GenEq/ml) #

Spn-FLUO

HN-1

5.73E + 05

+++

HN-2

1.67E + 06

+++

HN-3

1.61E + 04

+

HN-4

2.60E + 06

++

HN-5

1.46E + 04

++

HN-6

4.47E + 06

++

HN-7

1.20E + 06

+++

HN-8

1.49E + 05

++

HN-9

4.70E + 05

++

HN-10

2.72E + 05

+++

HN-11

1.75E + 04

ND

HN-12

1.51E + 04

++

HN-13

1.26E + 06

+++

HN-14

5.83E + 05

++

HN-15

1.08E + 05

+++

HN-16

1.87E + 04

++

HN-17

8.65E + 05

++

HN-18

2.92E + 06

+++

HN-19

3.81E + 05

+

HN-20

1.67E + 05

++

HN-21

4.18E + 05

++

HN-22

1.08E + 05

+

HN-23

1.12E + 05

+

HN-24

7.64E + 06

++

HN-25

8.88E + 06

++

HN-26 to HN-50

Negative

Negative

  1. #lytA-based qPCR assay. ND = not detected. (+) = 1–5 pneumococci per field, (++) = 5–10 pneumococci per field, (+++) = >10 pneumococci per field. Negative = no pneumococci observed